Publications

2025

Comprehensive interrogation of synthetic lethality in the DNA damage response

Fielden J, Siegner MS, Gallagher DN, Schröder MS, Dello Stritto MR, Lam S, Kobel L, Schlapansky MF, Jackson SP, Cejka P, Jost M, Corn JE.

Nature. 2025 Apr 9. doi: 10.1038/s41586-025-08815-4. Online ahead of print. PMID: 40205037

C-terminal amides mark proteins for degradation via SCF–FBXO31

Muhar MF, Farnung J, Cernakova M, Hoffman R, Henneberg LT, Pfleider MM, Denoth-Lippuner A, Kalčic F, Nievergelt AS, Peters Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek M, Schulman BA, Bode JW, Corn JE.

Nature. 2025 Jan 29. doi: 10.1038/s41586-024-08475-w. Online ahead of print. PMID: 39880951 

KLF5 loss sensitizes cells to ATR inhibition and is synthetic lethal with ARID1A deficiency

Awwad SW, Doyle C, Coulthard J, Bader AS, Gueorgueieva N, Lam S, Gupta V, Tran T-A, Balasubramanian S, Belotserkovskaya R, Jackson SP

Nat Commun. 2025 Jan 8;16(1):480. doi: 10.1038/s41467-024-55637-5. PMID: 39779698 PMCID: PMC11711288

2024

TDP1 splice-site mutation causes HAP1 cell hypersensitivity to topoisomerase I inhibition

Goh CG, Bader AS, Tran T-A, Belotserkovskaya R, D'Alessandro G, Jackson SP

Nucleic Acids Res. 2024 Dec 11:gkae1163. doi: 10.1093/nar/gkae1163. PMID: 39660638

Transcription-coupled repair of DNA-protein crosslinks

Carnie JC, Jackson SP, Stingele J

Trends Cell Biol. 2024 Nov 30:S0962-8924(24)00234-4. doi: 10.1016/j.tcb.2024.11.003. PMID: 39617652

Genome-aware annotation of CRISPR guides validates targets in variant cell lines and enhances discovery in screens

Lam S, Thomas JC, Jackson SP 

Genome Med. 2024 Nov 26;16(1):139. doi: 10.1186/s13073-024-01414-4. PMID: 39593080 PMCID: PMC11590575.

Genome editing with HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations.

Cullot G, Aird EJ, Schlapansky MF, Yeh CD, van de Venn L, Vykhlyantseva I, Kreutzer S, Mailänder D, Lewków B, Klermund J, Montellese C, Biserni M, Aeschimann F, Vonarburg C, Gehart H, Cathomen T, Corn JE.
Nat Biotechnol. 2024 Nov 27. doi: 10.1038/s41587-024-02488-6. PMID: 39604565.

Decitabine cytotoxicity is promoted by dCMP deaminase DCTD and mitigated by SUMO-dependent E3 ligase TOPORS

Carnie JC, Götz MJ,  Palma-Chaundler CS, Weickert P, Wanders A, Serrano-Benitez A, Li H-Y, Gupta V, Awwad SW, Blum CJ, Sczaniecka-Clift M, Cordes J, Zagnoli-Vieira G, D’Alessandro G, Richards SL, Gueorguieva N, Lam S, Beli P,  Stingele J, Jackson SP 

EMBO J. 2024 Jun;43(12):2397-2423. doi: 10.1038/s44318-024-00108-2. PMID: 38760575 PMCID: PMC11183266.

Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB

Carnie JC, Acampora CA, Bader AS, Erdenebat C, Zhao S, Bitensky E, van den Heuvel D, Parnas A, Gupta V, D'Alessandro G,  Sczaniecka-Clift M, Weickert P, Aygenli F, Götz MJ, Cordes J, Esain-Garcia I, Melidis L, Wondergem AP, Lam S, Robles MS, Balasubramanian S, Adar S, Luijsterburg MS, Jackson SP & Stingele J

Nat Cell Biol. 2024 May;26(5):797-810. doi: 10.1038/s41556-024-01391-1.  PMID: 38600235 PMCID: PMC11098753.

Removal of TREX1 activity enhances CRISPR-Cas9-mediated homology recombination.

Karasu ME, Toufectchan E, Chen Y, Albertelli A,  Cullot G, Maciejowski J, Corn JE
Nat Biotechnol. 2024 Aug 12. doi: 10.1038/s41587-024-02356-3. Online ahead of print. PMID: 39134754.

CCAR1 promotes DNA repair via alternative splicing

Karasu ME, Jahnke L, Joseph BJ, Amerzhanova Y, Mironov A, Shu X, Schröder MS, Ana Gvozdenovic A, Sala I, Zavolan M, Jonas S, Corn JE

Mol Cell. 2024 Jul 2:S1097-2765(24)00514-8. doi: 10.1016/j.molcel.2024.06.011. Online ahead of print. PMID: 38964321.

2023

RAD54L2 counters TOP2-DNA adducts to promote genome stability 

D'Alessandro G, Morales-Juarez DA, Richards SL, Nitiss KC, Serrano-Benitez A, Wang J, Thomas JC, Gupta V, Voigt A, Belotserkovskaya R, Goh CG, Bowden AR, Galanty Y, Beli P, Nitiss JL, Zagnoli-Vieira G, Jackson SP

Sci Adv. 2023 Dec 8;9(49):eadl2108. doi: 10.1126/sciadv.adl2108. PMID: 38055822. PMCID: PMC10699776.

Unrepaired base excision repair intermediates in template DNA strands trigger replication fork collapse and PARP inhibitor sensitivity

Serrano-Benitez A, Wells SE, Drummond-Clarke L, Russo LC,  Thomas JC, LEal GA, Farrow M, Edgerton JM, Balasubramanian S, Yang M, Frezza C, Gautam A, Brazina J, Burdova K, Hoch NC, Gupta V, Jackson SP, Caldecott KW

EMBO J. 2023 Sep 18;42(18):e113190. doi: 10.15252/embj.2022113190. PMID: 37492888. PMCID: PMC10505916. 

  Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens 

   Awwad SW, Serrano-Benitez A,  Thomas JC, Gupta V, Jackson SP

Nat Rev Mol Cell Biol.  2023 Feb 13. doi: 10.1038/s41580-022-00571-x. PMID: 36781955.

2022

Clinical prospects of WRN inhibition as a treatment for MSI tumours 

Morales-Juarez DA,  Jackson SP

NPJ Precis Oncol. 2022 Nov 15;6(1):85. doi: 10.1038/s41698-022-00319-y. PMID: 36379964; PMCID: PMC666358.

POLθ processes ssDNA gaps and promotes replication fork progression in BRCA1-deficient cells 

Schrempf A, Bernardo S, Arasa-Verge EA, Ramirez Otero MA, Wilson J, Kirchofer D, Timelthaler G, Ambros AM, Kaya A, Wieder M, Ecker GF, Winter GE, Costanzo V,  Loizou JI

Cell Rep. 2022 Nov 29;41(9):111716. doi: 10.1016/j.celrep.2022.111716. PMID: 36400033; PMCID: PMC9356142.

Recursive Editing improves homology-directed repair through retargeting of undesired outcomes 

Möller L, Aird EJ, Schröder MS, Kobel L, Kissling L, van den Venn L, Corn JE

Nature Commun. 2022 Aug 5;13(1):4550. doi: 10.1038/s41467-022-31944-7. Epub ahead of print. PMID: 35931681.

Clickable Cisplatin Derivatives as Versatile Tools to Probe the DNA Damage Response to Chemotherapy

Moretton A, Slyskova J, Simaan ME, Arasa-Verge EA, Meyenberg M, Cerrón-Infantes DA, Unterlass MM, Loizou JI 

Front Oncol. 2022 Jun 2;12:874201. doi: 10.3389/fonc.2022.874201. PMID: 35719993; PMCID: PMC9202558.

Exploring the genetic space of the DNA damage response for cancer therapy through CRISPR-based screens

Wilson J, Loizou JI 

Mol Oncol. 2022 Jun 16. doi: 10.1002/1878-0261.13272. Epub ahead of print. PMID: 35708734.

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

Ferreira da Silva J, Oliveira GP, Arasa-Verge EA, Kagiou C, Moretton A, Timelthaler G, Jiricny J, Loizou JI

Nat Commun. 2022 Feb 9;13(1):760. doi: 10.1038/s41467-022-28442-1. PMID: 35140211; PMCID: PMC8828784.

2020

Targeting the DNA Repair Enzyme Polymerase θ in Cancer Therapy

Schrempf A, Slyskova J, Loizou JI

Trends Cancer. 2021 Feb;7(2):98-111. doi: 10.1016/j.trecan.2020.09.007. Epub 2020 Oct 24. PMID: 33109489.